Amendment on the first lab handout

Perhaps most of you guys has already change what needed to be changed. Just to make sure that everyone got it, on the page 39 (the second page), please change 'O.D' to 'absorbance'. Whatever 'O.D' means, don't bother. Just focus on the protein electrophoresis, especially on the general idea; and of course the clinical importance. Remember the assignments that we should be prepared with for the whole 2 part of experiments?

1. Describe various factors that influence rate of migration of particles in electrophoresis;

2. Describe applications of electrophoresis in medicine;

3. Describe pattern of serum protein electrophoresis in, a) Multiple myeloma; b) Nephrotic syndrome, and; c) acute and chronic inflammation. The hint, observe which protein fraction elevated or decreased in conditions above;

4. Calculate absorbance of albumin and absorbance of total globulin fraction. Also, find out about normal A to G ratio and what causes low ratio.

Have a nice day :)

ps. Any mistakes, kindly leave comments :)

Addition from Farisya,

ACTUAL ABSORBANCE=ABSORBANCE OF TEST-ABSORBANCE OF BLANK

That's the formula for the experiment :) Thanks Farisya.